Plasmid_Backbone
Part:BBa_K4284004:Design
Designed by: Zhang Xinchi Group: iGEM22_PINGHE (2022-08-11)
pCDFD-bktB-ydil-backbone
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1241
Illegal XbaI site found at 3355
Illegal PstI site found at 329
Illegal PstI site found at 968
Illegal PstI site found at 1260 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1241
Illegal NheI site found at 2555
Illegal PstI site found at 329
Illegal PstI site found at 968
Illegal PstI site found at 1260
Illegal NotI site found at 1278 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1241
Illegal BamHI site found at 1235
Illegal XhoI site found at 1343 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1241
Illegal XbaI site found at 3355
Illegal PstI site found at 329
Illegal PstI site found at 968
Illegal PstI site found at 1260 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1241
Illegal XbaI site found at 3355
Illegal PstI site found at 329
Illegal PstI site found at 968
Illegal PstI site found at 1260
Illegal NgoMIV site found at 1808
Illegal AgeI site found at 1112
Illegal AgeI site found at 1555 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
NO
Source
NO